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polyclonal goat anti-human–fh ab  (Millipore)


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    Millipore polyclonal goat anti-human–fh ab
    Polyclonal Goat Anti Human–Fh Ab, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-human+fh+ab/horseradish+peroxidase/pm23863906-79-12-16
    Average 90 stars, based on 1 article reviews
    polyclonal goat anti-human–fh ab - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Lysine-dependent multipoint binding of the Borrelia burgdorferi virulence factor outer surface protein E to the C terminus of factor H.
    Article Snippet: The membranes were blocked with 5% (w/v) dried milk in PBS for 30 min and incubated at room temperature for 1 h with polyclonal goat anti-human FH Ab (Calbiochem, La Jolla, CA; diluted 1/5000).



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    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using <t>polyclonal</t> anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.
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    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using <t>polyclonal</t> anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.
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    polyclonal goat anti-human–fh ab  (Millipore)
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    Millipore polyclonal goat anti-human–fh ab
    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using <t>polyclonal</t> anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.
    Polyclonal Goat Anti Human–Fh Ab, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-human+fh+ab/horseradish+peroxidase/pm23863906-79-12-16
    Average 90 stars, based on 1 article reviews
    polyclonal goat anti-human–fh ab - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    polyclonal goat anti-human fh ab  (Millipore)
    90
    Millipore polyclonal goat anti-human fh ab
    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using <t>polyclonal</t> anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.
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    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using polyclonal anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.

    Journal: Frontiers in Immunology

    Article Title: Complement Factor H Family Proteins Modulate Monocyte and Neutrophil Granulocyte Functions

    doi: 10.3389/fimmu.2021.660852

    Figure Lengend Snippet: FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using polyclonal anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.

    Article Snippet: Bound proteins were detected via incubation with the polyclonal goat anti-human FH Ab in DPBS containing fetal bovine serum (FBS, 0.1%, EuroClone, Pero, Italy) for 15 minutes at 4°C, washed and labelled with Alexa-488-conjugated rabbit anti-goat antibody (Thermo Scientific, Waltham, MA) under the same conditions.

    Techniques: Binding Assay, Flow Cytometry, Incubation